Clonality analysis by methylation-specific PCR for the human androgen-receptor gene (HUMARA-MSP)
نویسندگان
چکیده
منابع مشابه
Methylation patterns in human androgen receptor gene and clonality analysis.
Tumor clonality, an important issue in tumor biology, has been analyzed using X-chromosome inactivation studies based on the differential methylation patterns of active and inactive alleles. Recently, a PCR-based androgen receptor gene (AR) analysis method was developed that takes advantage of highly polymorphic CAG repeats and nearby HpaII and HhaI sites in exon 1 of AR at the Xq13 region. How...
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Lambert Busque, Jiguang Zhu, David DeHart, Barbara Griffith, Cheryl Willman, Rona Carroll, Peter McL.BIack and D.G.Gilliland* Division of Hematology/Oncology, Department of Medicine, 1 Neurosurgical Laboratories and Brain Tumor Center, Brigham and Women's Hospital and Harvard Medical School, Boston, MA and Center for Molecular and Cellular Diagnostics, The University of New Mexico, Albuquerque,...
متن کاملClonality Analysis Methylation Patterns in Human Androgen Receptor Gene and Updated Version
Tumor clonality, an important issue in tumor biology, has been analyzed using X-chromosome inactivation studies based on the differential methylation patterns of active and inactive alleles. Recently, a PCR-based androgen receptor gene (AR) analysis method was developed that takes advantage of highly polymorphic CAG repeats and nearby HpaII and HhaI sites in exon 1 of AR at the Xq13 region. How...
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We assessed the feasibility of clonality analysis with human androgen receptor gene polymerase chain reaction in terms of the sensitivity and specificity for normal and cancerous colonic tissues taken from fourteen informative cases selected from 22 women with colonic adenocarcinoma. Ten crypts microdissected from each 10-microm-thick cryostat sections and whole tissues were used as samples. DN...
متن کاملMSRE-PCR for analysis of gene-specific DNA methylation
Abnormal DNA methylation is observed in certain promoters of neoplastic cells, although the likelihood of methylation for each individual promoter varies. Simultaneous analysis of many promoters in the same sample can allow use of statistical methods for identification of neoplasia. Here we describe an assay for such analysis, based on digestion of genomic DNA with methylation-sensitive restric...
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ژورنال
عنوان ژورنال: Leukemia
سال: 2000
ISSN: 0887-6924,1476-5551
DOI: 10.1038/sj.leu.2401631